Difference between revisions of "Part:BBa K538210"

 
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<partinfo>BBa_K538210 Icon</partinfo>
  
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===Usage and Biology===
 
  
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This part was submitted by team [http://2011.igem.org/Team:Amsterdam Amsterdam 2011] as part of their project: '''''icE. coli'''''. Its purpose is to express the ''Cpn60/10'' system from ''Polaribacter irgensii'', by simultaneously expressing ''Cpn10'' and ''Cpn60'' ([[Part:BBa_K538000|BBa_K538000]] and [[Part:BBa_K538001|BBa_K538001]], respectively). When expressed individually, these chaperones haven't been observed to significantly affect ''E. coli'', but when expressed together, they strongly increase ''E. coli'''s growth rate at suboptimal temperatures. Team Amsterdam submitted 4 operon-like Bricks that facilitate ''Cpn60/10'' coexpression. These are:
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<span class='h3bb'>Sequence and Features</span>
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* [[Part:BBa_K538210|BBa_K538210]]: pLacI + RBS + Cpn10 + RBS + Cpn60
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* [[Part:BBa_K538211|BBa_K538211]]: pLacI + RBS + Cpn60 + RBS + Cpn10
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* [[Part:BBa_K538310|BBa_K538310]]: pBAD + RBS + Cpn10 + RBS + Cpn60
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* [[Part:BBa_K538311|BBa_K538311]]: pBAD + RBS + Cpn60 + RBS + Cpn10
  
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The first two are controlled by the Lac operon's promoter, ''pLacI'' ([[Part:BBa_R0010|BBa_R0010]]). The latter two are placed under control of a strongly arabinose-induced promoter, derived from pBAD by team [http://2010.igem.org/Team:British_Columba British Columbia 2009].
  
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===Functional Parameters===
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Refer to the main pages of this part's coding regions (''Cpn10'': [[Part:BBa_K538000|BBa_K538000]] and ''Cpn60'': [[Part:BBa_K538001|BBa_K538001]]) for more information on how their expression facilitates cold resistance in ''E. coli''.
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<partinfo>BBa_K538210 parameters</partinfo>
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<partinfo>BBa_K538210 SequenceAndFeatures</partinfo>

Revision as of 18:35, 19 September 2011

Cpn10/60 operon (pLacI)

Cpn10/60
K538210


This part was submitted by team [http://2011.igem.org/Team:Amsterdam Amsterdam 2011] as part of their project: icE. coli. Its purpose is to express the Cpn60/10 system from Polaribacter irgensii, by simultaneously expressing Cpn10 and Cpn60 (BBa_K538000 and BBa_K538001, respectively). When expressed individually, these chaperones haven't been observed to significantly affect E. coli, but when expressed together, they strongly increase E. coli's growth rate at suboptimal temperatures. Team Amsterdam submitted 4 operon-like Bricks that facilitate Cpn60/10 coexpression. These are:

  • BBa_K538210: pLacI + RBS + Cpn10 + RBS + Cpn60
  • BBa_K538211: pLacI + RBS + Cpn60 + RBS + Cpn10
  • BBa_K538310: pBAD + RBS + Cpn10 + RBS + Cpn60
  • BBa_K538311: pBAD + RBS + Cpn60 + RBS + Cpn10

The first two are controlled by the Lac operon's promoter, pLacI (BBa_R0010). The latter two are placed under control of a strongly arabinose-induced promoter, derived from pBAD by team [http://2010.igem.org/Team:British_Columba British Columbia 2009].


Refer to the main pages of this part's coding regions (Cpn10: BBa_K538000 and Cpn60: BBa_K538001) for more information on how their expression facilitates cold resistance in E. coli.



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1419
    Illegal BamHI site found at 1485
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 278