Difference between revisions of "Part:BBa K553001:Design"

 
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<partinfo>BBa_K553001 short</partinfo>
 
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===Design Notes===
 
===Design Notes===
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this coding sequence become a standard Biobrick via per without any particular problems
+
Obtained via PCR from Agrobacterium tumefaciens gDNA
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 +
TraI fw
 +
 
 +
5' GTTTCTTCGAATTCGCGGCCGCTTCTAGATGCTGATTCTGACCGTCTCGCC 3'
 +
 
 +
TraI rev
 +
 
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5' GTTTCTTCCTGCAGCGGCCGCTACTAGTATCACGCCGCACTCCTCAACG 3'
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 +
 
  
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pcr protocol:
  
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95° 5' | 10x (93° 30" | 56° 30" | 72° 40") | 23x ( (93° 30" | 65° 30" | 72° 40") | 72° 7' | 4° ∞
  
 
===Source===
 
===Source===

Revision as of 16:45, 19 September 2011

A. tumefaciens TraI - OC8 HLA synthase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 385
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Obtained via PCR from Agrobacterium tumefaciens gDNA

TraI fw

5' GTTTCTTCGAATTCGCGGCCGCTTCTAGATGCTGATTCTGACCGTCTCGCC 3'

TraI rev

5' GTTTCTTCCTGCAGCGGCCGCTACTAGTATCACGCCGCACTCCTCAACG 3'


pcr protocol:

95° 5' | 10x (93° 30" | 56° 30" | 72° 40") | 23x ( (93° 30" | 65° 30" | 72° 40") | 72° 7' | 4° ∞

Source

http://www.ncbi.nlm.nih.gov/nucleotide/81176483?report=genbank&log$=nuclalign&blast_rank=1&RID=79664K7A015

References