Difference between revisions of "Part:BBa K538203"
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This part was submitted by team [http://2011.igem.org/Team:Amsterdam Amsterdam 2011] as part of their project: '''''icE. coli'''''. Its purpose is to express the ''CspA'' protein from ''Polaribacter irgensii'' ([[Part:BBa_K538003|BBa_K538003]]) under control of the Lac operon's promoter, ''pLacI'' ([[Part:BBa_R0010|BBa_R0010]]). ''E. coli'' expressing this protein experienec a drastic increase in their ability to survive freezing and thawing. This Brick's designers intend to use it to culture 'normal' ''E. coli'' faster at temperatures below its optimum (37°C), possibly even facilitating growth below the minimal growth temperature of 7°C.
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This part was submitted by team [http://2011.igem.org/Team:Amsterdam Amsterdam 2011] as part of their project: '''''icE. coli'''''. Its purpose is to express the ''CspA'' protein from ''Polaribacter irgensii'' ([[Part:BBa_K538003|BBa_K538003]]) under control of the Lac operon's promoter, ''pLacI'' ([[Part:BBa_R0010|BBa_R0010]]). ''E. coli'' expressing this protein experience a drastic increase in their ability to survive freezing and thawing. This Brick's designers intend to use it to culture 'normal' ''E. coli'' faster at temperatures below its optimum (37°C), possibly even facilitating growth below the minimal growth temperature of 7°C.
  
  

Revision as of 15:35, 19 September 2011

pLacI + RBS + CspA

LacI:CspA
K538203


This part was submitted by team [http://2011.igem.org/Team:Amsterdam Amsterdam 2011] as part of their project: icE. coli. Its purpose is to express the CspA protein from Polaribacter irgensii (BBa_K538003) under control of the Lac operon's promoter, pLacI (BBa_R0010). E. coli expressing this protein experience a drastic increase in their ability to survive freezing and thawing. This Brick's designers intend to use it to culture 'normal' E. coli faster at temperatures below its optimum (37°C), possibly even facilitating growth below the minimal growth temperature of 7°C.


Refer to the main pages of the coding region (CspA: BBa_K538003) for more information on how CspA facilitates cold resistance in E. coli.



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]