Difference between revisions of "Part:BBa K584024"

Revision as of 15:17, 19 September 2011

Ice Nucleating Protein Generator (RBS + InaZ [K584021] + Terminator)

This part is the Ice Nucleating protien generator. It is composed of the ribosome binding site (RBS), ice nucleating protein gene inaZ and the two terminators BBa_B0010 BBa_B0012.

The ice nucleation protein (INP) is a glycosyl phosphatidylinositol anchored outer membrane protein found in certain Gram-negative bacteria. inaZ is the gene responsible for producing the ice nucleation protein.

We would like to thank [http://www.academicbiography.uwo.ca/profile.php?&n=ggloor Prof. Dr. Gregory Gloor ], for providing us with a plasmid (pUC1813ICE) which contains the ice nucleating protein gene inaZ.

To know more about the inaZ, it is suggested to read this [http://www.ncbi.nlm.nih.gov/pubmed/10933828 article] describing the usage of pUC1813ICE with the inaZ.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 664
Illegal NgoMIV site found at 2176
Illegal NgoMIV site found at 2320
Illegal NgoMIV site found at 2584
Illegal NgoMIV site found at 2728
Illegal NgoMIV site found at 2776
Illegal NgoMIV site found at 2896
Illegal AgeI site found at 435
Illegal AgeI site found at 1840
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 610
Illegal BsaI.rc site found at 3356
Illegal SapI.rc site found at 256

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K584024 short</partinfo>
-This part is the Ice Nucleating protien generator. It is commposed of the ribosome binding site  [https://parts.igem.org/wiki/index.php?title=Part:BBa_B0034 (RBS)], ice nucleating protein gene  [https://parts.igem.org/wiki/index.php?title=Part:BBa_K584021 inaZ] and the two terminators [https://parts.igem.org/wiki/index.php?title=Part:BBa_B0010 BBa_B0010] [https://parts.igem.org/wiki/index.php?title=Part:BBa_B0012 BBa_B0012].
+This part is the Ice Nucleating protien generator. It is composed of the ribosome binding site  [https://parts.igem.org/wiki/index.php?title=Part:BBa_B0034 (RBS)], ice nucleating protein gene  [https://parts.igem.org/wiki/index.php?title=Part:BBa_K584021 inaZ] and the two terminators [https://parts.igem.org/wiki/index.php?title=Part:BBa_B0010 BBa_B0010] [https://parts.igem.org/wiki/index.php?title=Part:BBa_B0012 BBa_B0012].
 The ice nucleation protein (INP) is a glycosyl phosphatidylinositol anchored outer membrane protein found in certain Gram-negative bacteria. '''inaZ''' is the gene responsible for producing the ice nucleation protein.