Difference between revisions of "Part:BBa K538301"

Latest revision as of 14:50, 19 September 2011

pBAD + RBS + Cpn60

pBAD:Cpn60 K538301

This part was submitted by team [http://2011.igem.org/Team:Amsterdam Amsterdam 2011] as part of their project: icE. coli. Its purpose is to express the Cpn10 protein from Oleispira antarctica (BBa_K538000) under control of the strong arabinose-induced promoter team [http://2009.igem.org/Team:British_Columbia British Columbia 2009] derived from pBAD (BBa_K206000). When coexpressed with Cpn10 (BBa_K538000), it should strongly increase E. coli 's ability to grow at low temperature. Coexpression can be achieved by transforming an E. coli strain comprising this BioBrick with a Cpn10 generator, such as K538200 and K538300, or by transforming cells with an operon-like BioBrick containing both the Cpn10 and Cpn60 coding regions, such as K538210, K538211, K538310 and K538311.

Refer to the main pages of the coding region parts (Cpn10: BBa_K538000 and Cpn60: BBa_K538001) for more information on how they facilitate cold resistance in E. coli.

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 125
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1026
Illegal BamHI site found at 65
Illegal BamHI site found at 1092
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Revision as of 13:09, 19 September 2011 (view source) B.Stringer (Talk \| contribs) ← Older edit		Latest revision as of 14:50, 19 September 2011 (view source) B.Stringer (Talk \| contribs)
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−	This part was submitted by team [http://2011.igem.org/Team:Amsterdam Amsterdam 2011] as part of their project: '''''icE. coli'''''. Its purpose is to express the ''Cpn10'' protein from ''Oleispira antarctica'' ([[Part:BBa_K538000\|BBa_K538000]]) under control of the strong arabinose-induced promoter team [http://2009.igem.org/Team:British_Columbia British Columbia 2009] derived from pBAD ([[Part:BBa_K206000\|BBa_K206000]]). When coexpressed with ''Cpn10'' ([[Part:BBa_K538000\|BBa_K538000]]), it should strongly increase ''E. coli'' 's ability to grow at low temperature. Coexpression can be achieved by transforming an ''E. coli'' strain comprising this BioBrick with a ''Cpn10'' generator, such as [[Part:BBa_K538200\|K538200]] and [[Part:~~BBa_K538301~~\|~~K538301~~]], or by transforming cells with an operon-like BioBrick containing both the ''Cpn10'' and ''Cpn60'' coding regions, such as [[Part:BBa_K538210\|K538210]], [[Part:BBa_K538211\|K538211]], [[Part:BBa_K538310\|K538310]] and [[Part:BBa_K538311\|K538311]].	+	This part was submitted by team [http://2011.igem.org/Team:Amsterdam Amsterdam 2011] as part of their project: '''''icE. coli'''''. Its purpose is to express the ''Cpn10'' protein from ''Oleispira antarctica'' ([[Part:BBa_K538000\|BBa_K538000]]) under control of the strong arabinose-induced promoter team [http://2009.igem.org/Team:British_Columbia British Columbia 2009] derived from pBAD ([[Part:BBa_K206000\|BBa_K206000]]). When coexpressed with ''Cpn10'' ([[Part:BBa_K538000\|BBa_K538000]]), it should strongly increase ''E. coli'' 's ability to grow at low temperature. Coexpression can be achieved by transforming an ''E. coli'' strain comprising this BioBrick with a ''Cpn10'' generator, such as [[Part:BBa_K538200\|K538200]] and [[Part:BBa_K538300\|K538300]], or by transforming cells with an operon-like BioBrick containing both the ''Cpn10'' and ''Cpn60'' coding regions, such as [[Part:BBa_K538210\|K538210]], [[Part:BBa_K538211\|K538211]], [[Part:BBa_K538310\|K538310]] and [[Part:BBa_K538311\|K538311]].