Difference between revisions of "Part:BBa K538302"

 
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<partinfo>BBa_K538302 short</partinfo>
 
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<partinfo>BBa_K538302 Icon</partinfo>
  
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===Usage and Biology===
 
  
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This part was submitted by team [http://2011.igem.org/Team:Amsterdam Amsterdam 2011] as part of their project: '''''icE. coli'''''. Its purpose is to express the ''DnaK'' protein from ''Shewanella'' sp. AC10 ([[Part:BBa_K538002|BBa_K538002]]) under control of the strong arabinose-induced promoter team [http://2009.igem.org/Team:British_Columbia British Columbia 2009] derived from pBAD ([[Part:BBa_K206000|BBa_K206000]]). Heterologous expression of this protein has rescued ''E. coli DnaK''-null mutants, and facilitated their growth at 15°C. This Brick's designers intend to use it to culture 'normal' ''E. coli'' faster at temperatures below its optimum (37°C), possibly even facilitating growth below the minimal growth temperature of 7°C.
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K538302 SequenceAndFeatures</partinfo>
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Refer to the main pages of the coding region (''SheDnaK'': [[Part:BBa_K538002|BBa_K538002]]) for more information on how ''SheDnaK'' facilitates cold resistance in ''E. coli''.
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===Functional Parameters===
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<partinfo>BBa_K538302 parameters</partinfo>
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<partinfo>BBa_K538302 SequenceAndFeatures</partinfo>

Revision as of 13:39, 19 September 2011

pBAD + RBS + SheDnaK

pBAD:SDnaK
K538302


This part was submitted by team [http://2011.igem.org/Team:Amsterdam Amsterdam 2011] as part of their project: icE. coli. Its purpose is to express the DnaK protein from Shewanella sp. AC10 (BBa_K538002) under control of the strong arabinose-induced promoter team [http://2009.igem.org/Team:British_Columbia British Columbia 2009] derived from pBAD (BBa_K206000). Heterologous expression of this protein has rescued E. coli DnaK-null mutants, and facilitated their growth at 15°C. This Brick's designers intend to use it to culture 'normal' E. coli faster at temperatures below its optimum (37°C), possibly even facilitating growth below the minimal growth temperature of 7°C.


Refer to the main pages of the coding region (SheDnaK: BBa_K538002) for more information on how SheDnaK facilitates cold resistance in E. coli.



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 903
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]