Difference between revisions of "Part:BBa K553000:Design"
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The gene was extracted by pcr from Agrobacterium Tumefaciens gDNA. | The gene was extracted by pcr from Agrobacterium Tumefaciens gDNA. | ||
| − | http://www.ncbi.nlm.nih.gov/ | + | http://www.ncbi.nlm.nih.gov/gene/1224220 |
The sequencing revealed mutations in our strain that do not affect the part functionality. | The sequencing revealed mutations in our strain that do not affect the part functionality. | ||
===References=== | ===References=== | ||
Revision as of 13:30, 19 September 2011
TraR trans-activator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
the part was generated by using the following primers:
TraR fw
5' GTTTCTTCGAATTCGCGGCCGCTTCTAGATCGACGACTGGCTGGACAAGC 3'
TraR rev
5' GTTTCTTCCTGCAGCGGCCGCTACTAGTATCAGATGAGTTTCCGCCGGATG 3'
pcr protocol:
95° 5' | 10x (93° 30" | 56° 30" | 72° 40") | 23x ( (93° 30" | 65° 30" | 72° 40") | 72° 7' | 4° ∞
The part contains also the BBa_B0034 RBS and BBa_B0015 double terminator.
Source
The gene was extracted by pcr from Agrobacterium Tumefaciens gDNA.
http://www.ncbi.nlm.nih.gov/gene/1224220
The sequencing revealed mutations in our strain that do not affect the part functionality.