Difference between revisions of "Part:BBa K538200"
 
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Refer to the main pages of the coding region parts ([[Part:BBa_K538000|BBa_K538000]] and [[Part:BBa_K538001|BBa_K538001]]) for more information on how they facilitate cold resistance in ''E. coli''.
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Refer to the main pages of the coding region parts (''Cpn10'': [[Part:BBa_K538000|BBa_K538000]] and ''Cpn60'': [[Part:BBa_K538001|BBa_K538001]]) for more information on how they facilitate cold resistance in ''E. coli''.
  
  
 
<partinfo>BBa_K538200 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K538200 SequenceAndFeatures</partinfo>

Latest revision as of 13:09, 19 September 2011

pLacI + RBS + Cpn10

LacI:Cpn10
K538200


This part was submitted by team [http://2011.igem.org/Team:Amsterdam Amsterdam 2011] as part of their project: icE. coli. Its purpose is to express the Cpn10 protein from Oleispira antarctica (BBa_K538000) under control of the Lac operon's promoter, pLacI (BBa_R0010). When coexpressed with Cpn60 (BBa_K538001), it should strongly increase E. coli 's ability to grow at low temperature. Coexpression can be achieved by transforming an E. coli strain comprising this BioBrick with a Cpn60 generator, such as K538201 and K538301, or by transforming cells with an operon-like BioBrick containing both the Cpn10 and Cpn60 coding regions, such as K538210, K538211, K538310 and K538311.


Refer to the main pages of the coding region parts (Cpn10: BBa_K538000 and Cpn60: BBa_K538001) for more information on how they facilitate cold resistance in E. coli.



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 278