Difference between revisions of "Part:BBa K538200"

Revision as of 12:53, 19 September 2011

pLacI + RBS + Cpn10

LacI:Cpn10 K538200

This part was submitted by team [http://2011.igem.org/Team:Amsterdam Amsterdam 2011] as part of their project: icE. coli. Its purpose is to express the Cpn10 protein from Oleispira antarctica (BBa_K538000) under control of the Lac operon's promoter, pLacI (BBa_R0010). When coexpressed with Cpn60 (BBa_K538001), it should strongly increase E. coli 's ability to grow at low temperature. Coexpression can be achieved by transforming an E. coli strain comprising this BioBrick with a Cpn60 generator, such as K538201 and K538301, or by transforming cells with an operon-like BioBrick containing both the Cpn10 and Cpn60 coding regions, such as K538210, K538211, K538310 and K538311.

Refer to the main pages of the coding region parts (BBa_K538000 and BBa_K538001) for more information on how they facilitate cold resistance in E. coli.

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 278

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−	This part was submitted by team [http://2011.igem.org/Team:Amsterdam Amsterdam 2011] as part of their project: '''''icE. coli'''''. Its purpose is to express the ''Cpn10'' protein from ''Oleispira antarctica'' ([[Part:BBa_K538000\|BBa_K538000]]) under control of the Lac operon's promoter, ''pLacI'' ([[Part:BBa_R0010\|BBa_R0010]]). When coexpressed with ''Cpn60'' ([[Part:BBa_K538001\|BBa_K538001]]), it should strongly increase ''E. coli'' 's ability to grow at low temperature. Coexpression can be achieved by transforming an ''E. coli'' strain comprising ~~the~~ BioBrick with a ''Cpn60'' generator, such as [[Part:BBa_K538201\|K538201]] and [[Part:BBa_K538301\|K538301]], or by transforming cells with an operon-like BioBrick containing both the ''Cpn10'' and ''Cpn60'' coding regions, such as [[Part:BBa_K538210\|K538210]], [[Part:BBa_K538211\|K538211]], [[Part:BBa_K538310\|K538310]] and [[Part:BBa_K538311\|K538311]].	+	This part was submitted by team [http://2011.igem.org/Team:Amsterdam Amsterdam 2011] as part of their project: '''''icE. coli'''''. Its purpose is to express the ''Cpn10'' protein from ''Oleispira antarctica'' ([[Part:BBa_K538000\|BBa_K538000]]) under control of the Lac operon's promoter, ''pLacI'' ([[Part:BBa_R0010\|BBa_R0010]]). When coexpressed with ''Cpn60'' ([[Part:BBa_K538001\|BBa_K538001]]), it should strongly increase ''E. coli'' 's ability to grow at low temperature. Coexpression can be achieved by transforming an ''E. coli'' strain comprising this BioBrick with a ''Cpn60'' generator, such as [[Part:BBa_K538201\|K538201]] and [[Part:BBa_K538301\|K538301]], or by transforming cells with an operon-like BioBrick containing both the ''Cpn10'' and ''Cpn60'' coding regions, such as [[Part:BBa_K538210\|K538210]], [[Part:BBa_K538211\|K538211]], [[Part:BBa_K538310\|K538310]] and [[Part:BBa_K538311\|K538311]].