Difference between revisions of "Part:BBa K538130:Design"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K538130 short</partinfo>
 
<partinfo>BBa_K538130 short</partinfo>
  
<partinfo>BBa_K538130 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K538130 Icon</partinfo>
  
  
===Design Notes===
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This brick was used by team [http://2011.igem.org/Team:Amsterdam Amsterdam 2011] to create CryoBricks; cold resistance BioBricks. It serves as a construction intermediate for one or several of their protein generators. Neither of this brick's subparts was designed by the team, though; the incorporated promoter ([[Part:BBa_K206000|BBa_K20600]], submitted by [http://2009.igem.org/Team:British_Columbia British Columbia 2009]) and RBS ([[Part:BBa_B0034|BBa_B0034]]) were both taken from 2011's Spring Distribution Kit.
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For more information about the subparts' purpose and design, refer to their main page or design page, respectively.
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*'''BBa_K206000'''
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** [[Part:BBa_K206000 | Main Page]]
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** [[Part:BBa_K206000:Design | Design Page]]
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*'''BBa_B0034'''
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** [[Part:BBa_B0034 | Main Page]]
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** [[Part:BBa_B0034:Design | Design Page]]
  
===Source===
 
  
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<partinfo>BBa_K538130 SequenceAndFeatures</partinfo>
 
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===References===
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Latest revision as of 12:20, 19 September 2011

pBAD + RBS


K538130


This brick was used by team [http://2011.igem.org/Team:Amsterdam Amsterdam 2011] to create CryoBricks; cold resistance BioBricks. It serves as a construction intermediate for one or several of their protein generators. Neither of this brick's subparts was designed by the team, though; the incorporated promoter (BBa_K20600, submitted by [http://2009.igem.org/Team:British_Columbia British Columbia 2009]) and RBS (BBa_B0034) were both taken from 2011's Spring Distribution Kit.


For more information about the subparts' purpose and design, refer to their main page or design page, respectively.



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]