Difference between revisions of "Part:BBa K584015"

Revision as of 09:52, 19 September 2011

Lambda cI and LuxR regulated hybrid promoter + Ribolock-CeaB + Terminator

We performed a PCR to get the CeaB part of the K131009 biobrick. The primer design for this was such that it already contained the ribolock resulting in ‘Ribolock + ceaB’ as the final product. This is fused with the terminator B0015 to get ‘Ribolock + ceaB + B0015’. This was finally fused with the Lambda cI and LuxR regulated hybrid promotor to get this biobrick, ‘Lambda_cI and LuxR_regulated hybrid promotor’ + Ribolock + ceaB + B0015.

The primer sequence we used in the PCR reaction is -

ceaB-FW: CCGGAATTCGCGGCCGCTTCTAGAGAACTAGAATCACCTCTTGCTTTTGGGTAAGACGAAGAGGAGATACTAGATGAGCGGTGGCGATGGACGC

ceaB-RV: AAAACTGCAGCGGCCGCTACTAGTCTCTAGTATTACTTACCCCGATGAATATCAATAT

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K584015 short</partinfo>
-We performed a '''PCR''' to get the '''CeaB''' part of the '''K131009 biobrick'''. The primer design* for this was such that it already contained the ribolock resulting in ‘Ribolock + ceaB’ as the final product.  This is fused with the terminator B0015 to get ‘Ribolock + ceaB + B0015’. This was finally fused with the Lambda cI and LuxR regulated hybrid promotor to get  this biobrick, ''‘Lambda_cI and LuxR_regulated hybrid promotor’ + Ribolock + ceaB + B0015''.
+We performed a '''PCR''' to get the '''CeaB''' part of the '''K131009 biobrick'''. The primer design for this was such that it already contained the ribolock resulting in ‘Ribolock + ceaB’ as the final product.  This is fused with the terminator B0015 to get ‘Ribolock + ceaB + B0015’. This was finally fused with the Lambda cI and LuxR regulated hybrid promotor to get  this biobrick, ‘Lambda_cI and LuxR_regulated hybrid promotor’ + Ribolock + ceaB + B0015.
-*The primer sequence we used in the PCR reaction is -
+The primer sequence we used in the PCR reaction is -
 ceaB-FW:	CCGGAATTCGCGGCCGCTTCTAGAGAACTAGAATCACCTCTTGCTTTTGGGTAAGACGAAGAGGAGATACTAGATGAGCGGTGGCGATGGACGC