Difference between revisions of "Part:BBa K590016"

Revision as of 06:04, 19 September 2011

sfGFP_mamI_pGA1C3

This part was made by [http://2011.igem.org/Team:Washington UW iGEM Team 2011] and was contributed to the [http://2011.igem.org/Team:Washington/Magnetosomes/Results Magnetosome Toolkit].

Usage and Biology

This part consists of mamI gene from Magnetospirillum magneticum strain AMB-1, sfGFP in the backbone of pGA1C3. It was made by Gibson Cloning Method.

It was previously reported that the loss of mamI causes no membrane formation, and it functions as a localization protein onto chains.

This part was transformed into E.coli and membrane localization (particularly the poles) was observed:

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 80

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K590016 short</partinfo>
-Fluoroescent(sfGFP) membrane localization (mamI)
+This part was made by [http://2011.igem.org/Team:Washington UW iGEM Team 2011] and was contributed to the [http://2011.igem.org/Team:Washington/Magnetosomes/Results Magnetosome Toolkit].
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+This part consists of mamI gene from ''Magnetospirillum magneticum'' strain AMB-1, sfGFP in the backbone of [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590011 pGA1C3]. It was made by Gibson Cloning Method.
+It was previously reported that the loss of mamI causes no membrane formation, and it functions as a localization protein onto chains.
+This part was transformed into ''E.coli'' and membrane localization (particularly the poles) was observed:
+[[Image:SfGFP-I-1C3- uwigem2011.jpg|600px|center]]
 <!-- -->