Difference between revisions of "Part:BBa K528003"
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===Measurements=== | ===Measurements=== | ||
| + | Using this construct we have determined that strength of BBa_B0031 with RFP is 69,73% relative to BBa_B0034 with RFP. | ||
All our RFP measurement results are summarized on the chart below: | All our RFP measurement results are summarized on the chart below: | ||
Revision as of 21:51, 18 September 2011
RBS measurement device J23100 B0031 RFP
This device was used to determine BBa_B0031 strength relative to BBa_B0034.
Construct design
The device consists of BBa_K299501 expression vector J23100+B0031. E1010 RFP was used as reporter protein.
Authors
Clonning:
- Paweł Urbański
- Elżbieta Jankowska
- Krzysztof Szczepaniak
- Dorota Kaja Sabat
The lab work was supervised by:
- Michał Lower
- Ania Olchowik
Measurements
Using this construct we have determined that strength of BBa_B0031 with RFP is 69,73% relative to BBa_B0034 with RFP. All our RFP measurement results are summarized on the chart below:
For more info please look at Team Warsaw 2011 [http://2011.igem.org/Team:Warsaw/RBSmeasurement].
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 618
Illegal AgeI site found at 730 - 1000COMPATIBLE WITH RFC[1000]