Difference between revisions of "Part:BBa K528013"

Revision as of 21:42, 18 September 2011

RBS measurement device J23100 B0033 Super Folder GFP

This device was used to determine BBa_B0033 strength relative to BBa_B0034.

The device consists of BBa_K299503 expression vector J23100+B0033. BBa_I746916 SF-GFP was used as reporter protein.

Clonning:

The lab work was supervised by:

All our SF-GFP measurement results are summarized on the chart below:

For more info please look at Team Warsaw 2011 [http://2011.igem.org/Team:Warsaw/RBSmeasurement].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 73

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K528013 short</partinfo>
 This device was used to determine BBa_B0033 strength relative to BBa_B0034.
+===Construct design===
+The device consists of BBa_K299503 expression vector J23100+B0033. BBa_I746916 SF-GFP was used as reporter protein.
+===Authors===
+Clonning:
+*Paweł Urbański
+*Elżbieta Jankowska
+*Krzysztof Szczepaniak
+*Dorota Kaja Sabat
+The lab work was supervised by:
+*Michał Lower
+*Ania Olchowik
+===Measurements===
+All our SF-GFP measurement results are summarized on the chart below:
+https://static.igem.org/mediawiki/parts/5/5c/Wyk8.png
+For more info please look at Team Warsaw 2011 [http://2011.igem.org/Team:Warsaw/RBSmeasurement].
 <!-- Add more about the biology of this part here