Difference between revisions of "Part:BBa K523004"
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This is a version of <partinfo>BBa_K523002</partinfo> but with no RBS and no stop codon.
 
This is a version of <partinfo>BBa_K523002</partinfo> but with no RBS and no stop codon.
  
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There are four extra bases at the end which (together with the standard RFC10 prefix) generate a BglII site.
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There are four extra bases at the end which (together with the standard RFC10 prefix) generate a BglII site. There are two extra bases at the end which, when combined with the T at the start of the standard RFC10 suffix, code for a glycine. The effect of both of these features is to provide an in-frame BglII site at the start, and an in-frame SpeI site at the end (from the standard suffix). These features are necessary for producing fusion proteins with Edinburgh 2011's BioSandwich assembly protocol (<nowiki>RFC 81</nowiki>).
−
 
+
−
There are two extra bases at the end which, when combined with the T at the start of the standard RFC10 suffix, code for a glycine.
+
−
 
+
−
The effect of both of these features is to provide an in-frame BglII site at the start, and an in-frame SpeI site at the end (from the standard suffix). These features are necessary for Edinburgh 2011's BioSandwich assembly protocol.
+
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 20:14, 18 September 2011

bglX for BioSandwich

This is a version of BBa_K523002 but with no RBS and no stop codon.

There are four extra bases at the end which (together with the standard RFC10 prefix) generate a BglII site. There are two extra bases at the end which, when combined with the T at the start of the standard RFC10 suffix, code for a glycine. The effect of both of these features is to provide an in-frame BglII site at the start, and an in-frame SpeI site at the end (from the standard suffix). These features are necessary for producing fusion proteins with Edinburgh 2011's BioSandwich assembly protocol (RFC 81).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1625
    Illegal AgeI site found at 1847
    Illegal AgeI site found at 2036
  • 1000
    COMPATIBLE WITH RFC[1000]