Difference between revisions of "Part:BBa K510014:Design"
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===Design Notes=== | ===Design Notes=== | ||
+ | NcoI and SphI sites were added at the ends of the chloramphenicol resistance cassette to facilitate cloning | ||
===Source=== | ===Source=== |
Revision as of 19:49, 18 September 2011
pUC18R6KT-miniTn7BB-Km
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 4799
Illegal suffix found in sequence at 1 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 4799
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 4805 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 4799
Illegal BglII site found at 3212
Illegal BglII site found at 3483 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 4799
Illegal suffix found in sequence at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 4799
Illegal XbaI site found at 4814
Illegal SpeI site found at 2
Illegal PstI site found at 16 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1854
Illegal SapI site found at 518
Design Notes
NcoI and SphI sites were added at the ends of the chloramphenicol resistance cassette to facilitate cloning
Source
The pUC18R6KT-mini-Tn7-Km is a derivative of pUC18R6KT-mini-Tn7-Gm (BBa_K510012) by replacing the gentamycin resistance cassette by a kanamycin resistance cassette amplified from pSB4K5 with these primers: tatGCATGCCCATGGattggggctcactcaaagg and tatGCATGCCCATGGtcgacaatgtaactactag.