Difference between revisions of "Part:BBa K606029"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K606029 short</partinfo>
 
<partinfo>BBa_K606029 short</partinfo>
  
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This part is design to express the GFP in B. Subtilis.
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.This is a GFP expression system for B. Subtilis that is dont to be polymerazed by the T7 polymerase. This have to be activated by the pT7 promoter I712074. The RBS used is SpoVG, that is design for a strong expression in B. Subtilis.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 18:00, 18 September 2011

RBS SpoVG GFPmut3b T7terminator

.This is a GFP expression system for B. Subtilis that is dont to be polymerazed by the T7 polymerase. This have to be activated by the pT7 promoter I712074. The RBS used is SpoVG, that is design for a strong expression in B. Subtilis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 886
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 662