Difference between revisions of "Part:BBa K515010:Experience"

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<p>We have made a protein gel in order to verify whether the promoter is over-expressing XylE in the <a href="https://parts.igem.org/Part:BBa_K316005">BBa_K316005</a> construct in a DH5-α strain of <i>Escherichia coli</i>. The results showed a clear thick band corresponding to the correct size of the protein.</p>
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<I>Imperial College iGEM 2011</I>
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<p><i>Protein gel of XylE and control cells. Lane 1 contains the XylE producing <i>E. coli</i> and lane two contains the control cell line. The Ladder is a Fermentas PageRuler.</i></p>
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Revision as of 17:29, 18 September 2011

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K515010

  • The enzymatic reactions of the IAM pathway produce indole-3 acetic acid (IAA) which is an important phytohormone.
  • Many Plant Growth Promoting Rhizobacteria produce IAA
  • Analogues of phytohormones like α-Naphthaleneacetic acid are usually found in rooting powders. Therefore, if we were to express IAA in bacteria in a controlled fashion we could increase root growth.
  • Increased root growth could help maintain top soil.
  • Evidence has shown that using IAA on cotton can increase yields.


User Reviews

We have made a protein gel in order to verify whether the promoter is over-expressing XylE in the BBa_K316005 construct in a DH5-α strain of Escherichia coli. The results showed a clear thick band corresponding to the correct size of the protein.

Protein gel of XylE and control cells. Lane 1 contains the XylE producing E. coli and lane two contains the control cell line. The Ladder is a Fermentas PageRuler.