Difference between revisions of "Part:BBa K537016"
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<partinfo>BBa_K537016 short</partinfo> | <partinfo>BBa_K537016 short</partinfo> | ||
| − | + | This composite BioBrick begins with a strong, constitutively active promoter of E.coli (BBa_J23119) followed by an RBS which is fused to CheZ and monomeric Red fluorescent reporter protein (mRFP) without stop codons in between. The Freiberg iGEM 2007 BioBrick 3.0 fusion protein assembly was used to construct this BioBrick. The RBS-CheZ fusion was constructed via PCR. This part ends with a standard double terminator transcriptional terminator for E.coli (BBa_B0015). | |
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Revision as of 16:47, 18 September 2011
Promoter-RBS-CheZ-mCherry-Double terminator
This composite BioBrick begins with a strong, constitutively active promoter of E.coli (BBa_J23119) followed by an RBS which is fused to CheZ and monomeric Red fluorescent reporter protein (mRFP) without stop codons in between. The Freiberg iGEM 2007 BioBrick 3.0 fusion protein assembly was used to construct this BioBrick. The RBS-CheZ fusion was constructed via PCR. This part ends with a standard double terminator transcriptional terminator for E.coli (BBa_B0015).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1245
Illegal AgeI site found at 1357 - 1000COMPATIBLE WITH RFC[1000]