Difference between revisions of "Part:BBa K537010"
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<partinfo>BBa_K537010 short</partinfo> | <partinfo>BBa_K537010 short</partinfo> | ||
| − | + | This composite BioBrick begins with a strong, constitutively active promoter of E.coli (BBa_J23119) followed by a theophylline riboswitch (type2) (Lynch and Gallivan NAR 2009) which is fused to the Venus fluorescent reporter protein without a stop codon in between. The theophylline riboswitch2-venus fusion was constructed via 2 rounds of PCR. This part ends with a standard double terminator transcriptional terminator for E.coli (BBa_B0015). | |
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Revision as of 16:30, 18 September 2011
Promoter-Theophylline riboswitch 2-Venus-Double terminator
This composite BioBrick begins with a strong, constitutively active promoter of E.coli (BBa_J23119) followed by a theophylline riboswitch (type2) (Lynch and Gallivan NAR 2009) which is fused to the Venus fluorescent reporter protein without a stop codon in between. The theophylline riboswitch2-venus fusion was constructed via 2 rounds of PCR. This part ends with a standard double terminator transcriptional terminator for E.coli (BBa_B0015).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 730
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]