Difference between revisions of "Part:BBa K584004"
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<partinfo>BBa_K584004 short</partinfo>
 
<partinfo>BBa_K584004 short</partinfo>
  
−
This part was designed to test the functionality of the promotor region. we cloned the HybB promoter via PCR using the [https://parts.igem.org/Part:BBa_K410000 K410000] as a template using the primers below -
+
This part was designed to test the functionality of the promotor region. We cloned the HybB promoter via PCR using the [https://parts.igem.org/Part:BBa_K410000 K410000] as a template using the primers below -
  
 
hyb-FW: CCGGAATTCGCGGCCGCTTCTAGAGCGCCGCTATGGACTGGATAAAG   
 
hyb-FW: CCGGAATTCGCGGCCGCTTCTAGAGCGCCGCTATGGACTGGATAAAG   

Revision as of 15:59, 18 September 2011

HybB promoter + GFP generator

This part was designed to test the functionality of the promotor region. We cloned the HybB promoter via PCR using the K410000 as a template using the primers below -

hyb-FW: CCGGAATTCGCGGCCGCTTCTAGAGCGCCGCTATGGACTGGATAAAG

hyb-RV: AAAACTGCAGCGGCCGCTACTAGTATGCTACTTAACCCCATGGTGG

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1064