Difference between revisions of "Part:BBa K584020"

Revision as of 15:00, 18 September 2011

AFP extracellular protein generator

This part contains BBa_K584019 [ OmpA_transmembrane_domain + Linker + point_mutant_HPLC6(AFP) ] with the B0034 RBS, B0015 Terminator and iGEM standard restriction sites EcoRI, NotI, XbaI and SpeI, NotI, PstI.

A point mutation at Base Pair number 156 of the HPLC6 sequence was performed by replacing the T in the wild-type coding sequence to C. This mutation was necessary as the sequence CTGCAG in the Wild-type coding sequence corresponded to a Pst1 restriction site. GCT(Wild type) and GCC(1 point mutant) are both valid triplet codons for Alanine.

Detailed information of the Pseudopleuronectes americanus Antifreeze Protein (HPLC gene) can be found on this NCBI [http://www.ncbi.nlm.nih.gov/nuccore/M62417 webpage].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Revision as of 13:02, 18 September 2011 (view source) Vinchhi (Talk \| contribs) m ← Older edit		Revision as of 15:00, 18 September 2011 (view source) Vinchhi (Talk \| contribs) Newer edit →
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	A point mutation at Base Pair number 156 of the HPLC6 sequence was performed by replacing the T in the wild-type coding sequence to C. This mutation was necessary as the sequence CTGCAG in the Wild-type coding sequence corresponded to a Pst1 restriction site. GCT(Wild type) and GCC(1 point mutant) are both valid triplet codons for Alanine.		A point mutation at Base Pair number 156 of the HPLC6 sequence was performed by replacing the T in the wild-type coding sequence to C. This mutation was necessary as the sequence CTGCAG in the Wild-type coding sequence corresponded to a Pst1 restriction site. GCT(Wild type) and GCC(1 point mutant) are both valid triplet codons for Alanine.

−	Detailed information of the Pseudopleuronectes americanus Antifreeze Protein (HPLC gene) can be found on this NCBI ~~webpage~~ http://www.ncbi.nlm.nih.gov/nuccore/M62417.1	+	Detailed information of the Pseudopleuronectes americanus Antifreeze Protein (HPLC gene) can be found on this NCBI [http://www.ncbi.nlm.nih.gov/nuccore/M62417 webpage].