Difference between revisions of "Part:BBa K584021"

Revision as of 14:17, 18 September 2011

Ice Nucleating Protein Gene - inaZ

This part contains the Ice nuceating protein coding sequence. The ice nucleation protein (INP) is a glycosyl phosphatidylinositol anchored outer membrane protein found in certain Gram-negative bacteria. inaZ is the gene repsonsible for producing the ice nucleation protein.

We would like to thank Prof. Dr. Gregory Gloor (http://www.academicbiography.uwo.ca/profile.php?&n=ggloor), for providing us with a plasmid (pUC1813ICE) which contains the ice nucleating protein gene inaZ.

An article in which he uses the pUC1813ICE with inaZ can be found at http://www.ncbi.nlm.nih.gov/pubmed/10933828.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 646
Illegal NgoMIV site found at 2158
Illegal NgoMIV site found at 2302
Illegal NgoMIV site found at 2566
Illegal NgoMIV site found at 2710
Illegal NgoMIV site found at 2758
Illegal NgoMIV site found at 2878
Illegal AgeI site found at 417
Illegal AgeI site found at 1822
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 592
Illegal BsaI.rc site found at 3338
Illegal SapI.rc site found at 238

@@ Line 7: / Line 7: @@
 An article in which he uses the pUC1813ICE with inaZ can be found at http://www.ncbi.nlm.nih.gov/pubmed/10933828.
-''References''
-[1] Bassi AS Et al., ''Expression of single chain antibodies (ScFvs) for c-myc oncoprotein in recombinant Escherichia coli membranes by using the ice-nucleation protein of Pseudomonas syringae''. Biotechnol Prog. 2000 Jul-Aug;16(4):557-63.
 <!-- Add more about the biology of this part here