Difference between revisions of "Part:BBa K553002:Design"
(→Design Notes) |
|||
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K553002 short</partinfo> | <partinfo>BBa_K553002 short</partinfo> | ||
| Line 7: | Line 6: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | the pcr amplification worked well, few mutation observed after sequencing that are present in our strain | + | |
| + | the pcr amplification worked well, few mutation observed after sequencing that are present in our strain | ||
| + | |||
| + | '''PromTraFw''' | ||
| + | |||
| + | 5' GTTTCTCGAATTCGCGGCCGCTTCTAGAGAAGTGCAGATTTGCACATGAAATCAACG 3' | ||
| + | |||
| + | '''PromTra rev"" | ||
| + | |||
| + | 5' GTTTCTTCCTGCAGCGGCCGCTACTAGTATAGCGGAGAGAAAACATCTCATTCAGG 3' | ||
| + | |||
| + | pcr protocol: | ||
| + | |||
| + | 95° 5' | 10x (93° 30" | 56° 30" | 72° 40") | 23x ( (93° 30" | 65° 30" | 72° 40") | 72° 7' | 4° ∞ | ||
| + | ---- | ||
===Source=== | ===Source=== | ||
Revision as of 13:56, 18 September 2011
PTraI - TraR-OC8 HLA complex regulated promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 105
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
the pcr amplification worked well, few mutation observed after sequencing that are present in our strain
PromTraFw
5' GTTTCTCGAATTCGCGGCCGCTTCTAGAGAAGTGCAGATTTGCACATGAAATCAACG 3'
PromTra rev""
5' GTTTCTTCCTGCAGCGGCCGCTACTAGTATAGCGGAGAGAAAACATCTCATTCAGG 3'
pcr protocol:
95° 5' | 10x (93° 30" | 56° 30" | 72° 40") | 23x ( (93° 30" | 65° 30" | 72° 40") | 72° 7' | 4° ∞
Source
http://www.ncbi.nlm.nih.gov/nucleotide/8572673?report=genbank&log$=nuclalign&blast_rank=1&RID=795BNUZN014