Difference between revisions of "Part:BBa K537007"

Latest revision as of 13:45, 18 September 2011

RBS CheZ N-Fusion

The RBS CheZ- Fusion BioBrick consists of a RBS fused to the E. coli CheZ motility factor that lacks a stop codon at the N-terminus (although a TAG stop codon is provided by the RFC 25 suffix). CheZ in this part contains the standard BioBrick prefix and the Freiburg (2007) fusion suffix. The N-terminal part can be fused to a reporter or other CDS. Using this BioBrick, CheZ is constitutively expressed.

CheZ is the chief regulator of the molecular events that lead the counter clockwise rotation of the flagella motor during the chemotaxis signal transduction pathway of E.coli. This counter clockwise flagella motor rotation results in bacterial swimming (instead of tumbling).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K537007 short</partinfo>
-The RBS CheZ- Fusion BioBrick consists of a RBS fused to the E. coli CheZ motility factor that lacks a stop codon at the N-terminus. CheZ in this part contains the standard BioBrick prefix and the Freiburg (2007) fusion suffix. Using this BioBrick CheZ is constitutively expressed.
+The RBS CheZ- Fusion BioBrick consists of a RBS fused to the E. coli CheZ motility factor that lacks a stop codon at the N-terminus (although a TAG stop codon is provided by the RFC 25 suffix). CheZ in this part contains the standard BioBrick prefix and the Freiburg (2007) fusion suffix. The N-terminal part can be fused to a reporter or other CDS.  Using this BioBrick, CheZ is constitutively expressed.
+CheZ is the chief regulator of the molecular events that lead the counter clockwise rotation of the flagella motor during the chemotaxis signal transduction pathway of E.coli. This counter clockwise flagella motor rotation results in bacterial swimming (instead of tumbling).
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