Difference between revisions of "Part:BBa K537008:Design"

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===Design Notes===
 
===Design Notes===
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none
 
  
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This biobrick part represents a composite of a atrazine riboswitch upstream of a mRFP1 fluorescent protein reporter (derived from part BBa_E1010).
  
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Previous teams: Lethbridge 2007, Lethbridge 2009, and NYMU Taipei 2010 all considered strategies for making the riboswitch a separate biobrick, however standard assembly techniques are inadequate since the sequence distance between the ATG, RBS and aptamer domain of the riboswitch are critical. We have explored this further in a more elaborate discussion here.
  
 
===Source===
 
===Source===

Revision as of 13:36, 18 September 2011

Atrazine Riboswitch-mRFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 9
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 640
    Illegal AgeI site found at 752
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This biobrick part represents a composite of a atrazine riboswitch upstream of a mRFP1 fluorescent protein reporter (derived from part BBa_E1010).

Previous teams: Lethbridge 2007, Lethbridge 2009, and NYMU Taipei 2010 all considered strategies for making the riboswitch a separate biobrick, however standard assembly techniques are inadequate since the sequence distance between the ATG, RBS and aptamer domain of the riboswitch are critical. We have explored this further in a more elaborate discussion here.

Source

The mRFP1 fluorescent reporter is derived from part BBa_E1010

References