Difference between revisions of "Part:BBa K527001:Design"

(→‎Source)
(→‎Design Notes)
Line 8: Line 8:
 
Mutated at a Pst1 restiction site (nucleotide 342)
 
Mutated at a Pst1 restiction site (nucleotide 342)
 
No prefix and suffix.
 
No prefix and suffix.
−
User assembly ends.  
+
User assembly (Plug 'n' Play) ends.
−
 
+
−
 
+
  
 
===Source===
 
===Source===

Revision as of 13:35, 18 September 2011

Biosynthesis/Oximes/CYP79A2/Plug 'n' Play


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 165
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 432


Design Notes

Mutated at a Pst1 restiction site (nucleotide 342) No prefix and suffix. User assembly (Plug 'n' Play) ends.

Source

Institute of Plant Biology and Biotechnology

Faculty of LIFE Science

University of Copenhagen

Denmark

http://2011.igem.org/Team:DTU-Denmark-2

References

  • Wittstock U and Halkier B A (2000). Cytochrome P450 CYP79A2 from Arabidopsis thaliana L. Catalyzes

the Conversion of L-Phenylalanine to Phenylacetaldoxime in the Biosynthesis of Benzylglucosinolate. The Journal of Biological Chemistry Vol. 275, No. 19, Issue of May 12, pp. 14659–14666.

  • Møller B L (2010). Dynamic Metabolons. Science Vol. 330, 1328-1329.