Difference between revisions of "Part:BBa K553000:Design"
(→Design Notes) |
(→Design Notes) |
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the part was generated by per with these primers: | the part was generated by per with these primers: | ||
| − | TraR rev 5' GTTTCTTCCTGCAGCGGCCGCTACTAGTATCAGATGAGTTTCCGCCGGATG 3' | + | '''TraR rev ''' |
| − | TraR fw 5' GTTTCTTCGAATTCGCGGCCGCTTCTAGATCGACGACTGGCTGGACAAGC 3' | + | |
| + | 5' GTTTCTTCCTGCAGCGGCCGCTACTAGTATCAGATGAGTTTCCGCCGGATG 3' | ||
| + | |||
| + | '''TraR fw ''' | ||
| + | |||
| + | 5' GTTTCTTCGAATTCGCGGCCGCTTCTAGATCGACGACTGGCTGGACAAGC 3' | ||
with the present protocol: | with the present protocol: | ||
Revision as of 13:34, 18 September 2011
TraR trans-activator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
the part was generated by per with these primers:
TraR rev
5' GTTTCTTCCTGCAGCGGCCGCTACTAGTATCAGATGAGTTTCCGCCGGATG 3'
TraR fw
5' GTTTCTTCGAATTCGCGGCCGCTTCTAGATCGACGACTGGCTGGACAAGC 3'
with the present protocol:
95° 5' | 10x (93° 30" | 56° 30" | 72° 40") | 23x ( (93° 30" | 65° 30" | 72° 40") | 72° 7' | 4° ∞
The part contains also the BBa_B0034 RBS and BBa_B0015 double terminator.
Source
The gene was extracted by pcr from Agrobacterium Tumefaciens gDNA.
http://www.ncbi.nlm.nih.gov/nucleotide/81176483?report=genbank&log$=nuclalign&blast_rank=1&RID=7BS4G4AC014.
The sequencing revealed mutations in our strain that do not affect the part functionality.