Difference between revisions of "Part:BBa K537000:Design"
(→Design Notes) |
(→Source) |
||
| Line 11: | Line 11: | ||
The CheZ gene was isolated from genomic DNA using PCR where the forward primers in the reaction contained the sequence for the atrazine riboswitch. | The CheZ gene was isolated from genomic DNA using PCR where the forward primers in the reaction contained the sequence for the atrazine riboswitch. | ||
| + | |||
| + | The mRFP1 fluorescent reporter is derived from part BBa_E1010 | ||
===References=== | ===References=== | ||
Sinha J., Reyes S.J., and Gallivan J.P. Reprogramming Bacteria to Seek and Destroy a Herbicide. 2010, Nat Chem Biol. 6(6): 464–470 | Sinha J., Reyes S.J., and Gallivan J.P. Reprogramming Bacteria to Seek and Destroy a Herbicide. 2010, Nat Chem Biol. 6(6): 464–470 | ||
Revision as of 13:28, 18 September 2011
Atrazine Riboswitch-CheZ fusion
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 9
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The effectiveness of the atrazine riboswitch is determined by the ability of the RNA 'loop' to hide the RBS preventing translation. The sequence of bases within the riboswitch will determine the formation of the loop and its leakiness.
Source
The CheZ gene was isolated from genomic DNA using PCR where the forward primers in the reaction contained the sequence for the atrazine riboswitch.
The mRFP1 fluorescent reporter is derived from part BBa_E1010
References
Sinha J., Reyes S.J., and Gallivan J.P. Reprogramming Bacteria to Seek and Destroy a Herbicide. 2010, Nat Chem Biol. 6(6): 464–470