Difference between revisions of "Part:BBa K528000"

Revision as of 13:00, 18 September 2011

RBS measurement device J23100 B0031 mORANGE

The device consists of BBa_K299501 expression vector J23100+B0031 and mORANGE E2050 as a protein.

General info

In order to fine-tune expression of genes used in our project we have conducted measurement of various ribosome binding sites included in 2011 spring distribution. Our list of measured parts includes YFP E0030 (part 723 bp pSB1AK3), GFP E0020 (part 723bp pSB1A2), RFP E1010 (part 681 bp pSB2K3), mOrange E2050, MATSG—GSGTAlinkers (part 744bp pSB2K3), GFP E0040 GFPmut3b (720 bp pSB1A2). Also DNA of SUPERFOLDER GFP, which was included in this distribution as a bigger part with RBS and promotor. We obtained coding sequence of SUPERFOLDER GFP using PCR method.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 826
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K528000 short</partinfo>
 The device consists of BBa_K299501 expression vector J23100+B0031 and mORANGE E2050 as a protein.
+<b>General info</b><p align=justify> In order to fine-tune expression of genes used in our project we have conducted measurement of various ribosome binding sites included in 2011 spring distribution. Our list of measured parts includes YFP E0030 (part 723 bp  pSB1AK3), GFP E0020 (part 723bp   pSB1A2), RFP E1010 (part 681 bp  pSB2K3), mOrange E2050, MATSG—GSGTAlinkers (part 744bp   pSB2K3), GFP     E0040   GFPmut3b (720 bp pSB1A2). Also DNA of SUPERFOLDER GFP, which was included in this distribution as a bigger part with RBS and promotor. We obtained coding sequence of SUPERFOLDER GFP using PCR method.</p>
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