Difference between revisions of "Part:BBa K553000:Design"

(→‎Source)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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the part was generated by per with these primers:
  
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TraR rev 5' GTTTCTTCCTGCAGCGGCCGCTACTAGTATCAGATGAGTTTCCGCCGGATG  3'
 +
TraR fw 5' GTTTCTTCGAATTCGCGGCCGCTTCTAGATCGACGACTGGCTGGACAAGC    3'
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with the present protocol:
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 +
95° 5' | 10x (93° 30" | 56° 30" | 72° 40") | 23x ( (93° 30" | 65° 30" | 72° 40") | 72° 7' | 4° ∞
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The part contains also the BBa_B0034 RBS and BBa_B0015 double terminator.
  
 
===Source===
 
===Source===

Revision as of 12:21, 18 September 2011

TraR trans-activator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

the part was generated by per with these primers:

TraR rev 5' GTTTCTTCCTGCAGCGGCCGCTACTAGTATCAGATGAGTTTCCGCCGGATG 3' TraR fw 5' GTTTCTTCGAATTCGCGGCCGCTTCTAGATCGACGACTGGCTGGACAAGC 3'

with the present protocol:

95° 5' | 10x (93° 30" | 56° 30" | 72° 40") | 23x ( (93° 30" | 65° 30" | 72° 40") | 72° 7' | 4° ∞


The part contains also the BBa_B0034 RBS and BBa_B0015 double terminator.

Source

The gene was extracted by pcr from Agrobacterium Tumefaciens gDNA.

http://www.ncbi.nlm.nih.gov/nucleotide/81176483?report=genbank&log$=nuclalign&blast_rank=1&RID=7BS4G4AC014.

The sequencing revealed mutations in our strain that do not affect the part functionality.

References