Difference between revisions of "Part:BBa I13522:Experience"
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The characterization was performed by METU-TURKEY IGEM 2010 Team. | The characterization was performed by METU-TURKEY IGEM 2010 Team. | ||
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| + | <p><b>Thermostability Assay</b></p> | ||
| + | <p>For this BioBrick, the denaturation temperature was determined by heating the protein at a range of temperatures, and then measuring the fluorescence.</p> | ||
===Applications of BBa_I13522=== | ===Applications of BBa_I13522=== | ||
Revision as of 14:58, 17 September 2011
For parts BBa_I13521 and BBa_I13522, their excitation and emission maxima were determined. BBa_B0034 was used as a negative control. These data were used later in following characterization experiments as set parameters. The host was E. coli Top Ten in all experiments.
Meanwhile, the graph below shows the emission scan for GFP in part BBa_I13522. Since it was the best option in terms of fluorescence yield, 395 nm was set as the excitation wavelength. As it can be seen from the graph, maximum emission wavelength is 515 nm.
The characterization was performed by METU-TURKEY IGEM 2010 Team.
Thermostability Assay
For this BioBrick, the denaturation temperature was determined by heating the protein at a range of temperatures, and then measuring the fluorescence.
===Applications of BBa_I13522=== ===User Reviews===