Difference between revisions of "Part:BBa K525517"
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|rowspan="4"|Expression in ''E. coli'' | |rowspan="4"|Expression in ''E. coli'' | ||
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|Compatibility | |Compatibility | ||
|''E. coli'' KRX, TOP10, MACH1, BL21(DE3) | |''E. coli'' KRX, TOP10, MACH1, BL21(DE3) | ||
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|Working BPA concentration | |Working BPA concentration | ||
| − | | | + | |120 mg L<sup>-1</sup> (0.53 mM) |
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|rowspan="3"|Purification | |rowspan="3"|Purification | ||
Revision as of 13:35, 17 September 2011
Fusion protein of BisdA and BisdB behind constitutive promoter
Fusion protein of BisdA and BisdB behind constitutive promoter.
Usage and Biology
Expressing this BioBrick in E. coli enables the bacterium to degrade the endocrine disruptor bisphenol A (BPA).
Important parameters
| Experiment | Characteristic | Result |
|---|---|---|
| Expression in E. coli | Compatibility | E. coli KRX, TOP10, MACH1, BL21(DE3) |
| Expression | Constitutive | |
| Optimal temperature | 30 °C | |
| Working BPA concentration | 120 mg L-1 (0.53 mM) | |
| Purification | Molecular weight | 59.3 kDa |
| Theoretical pI | 4.99 | |
| High absorbation | 450 nm | |
| Degradation of BPA | Completely degradation of 0.53 mM BPA | 21 - 24 h |
| Specific BPA degradation rate | 1.29 10-10 mM cell-1 |
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 664
Illegal BamHI site found at 1402 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 65
Illegal AgeI site found at 1655 - 1000COMPATIBLE WITH RFC[1000]