Difference between revisions of "Part:BBa K590053"

Revision as of 18:49, 16 September 2011

RrD_PSB1C3-High constitutive

This part consists of Acyl-ACP Reductase, ribosomal binding site, Aldehyde Decarbonylase under the control of high consititutive promoter for alkane production.

Usage and Biology

Data collected from In Vivo Assay developed by University of Washington iGEM Team

When AAR and ADC are co-expressed, they produce odd chain length alkanes from the cell's fatty acid biosynthetic pathway. Alkane production is enhanced when growing expression strains using the growth conditions developed by the [http://2011.igem.org/Team:Washington 2011 University of Washington iGEM Team].

GC plot showing that alkanes are only produced when both ADC and AAR are expressed.

GC-MS quantification shows greater alkane production under UW iGEM 2011's "optimized conditions" (see wiki for details)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 470
Illegal NheI site found at 493
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 1584
Illegal XhoI site found at 2366
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 126
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 ===Usage and Biology===
+[[Image:Washington_Redesign_Data2.png|left|400px|thumb|Data collected from In Vivo Assay developed by University of Washington iGEM Team]]
+When AAR and ADC are co-expressed, they produce odd chain length alkanes from the cell's fatty acid biosynthetic pathway. Alkane production is enhanced when growing expression strains using the growth conditions developed by the [http://2011.igem.org/Team:Washington 2011 University of Washington iGEM Team].
+[[Image:Washinton_AARADCGC-MS.png|left|400px|thumb|GC plot showing that alkanes are only produced when both ADC and AAR are expressed.]]
+[[Image:Washinton_2011_Optimization_Quant.png|right|450px|thumb|GC-MS quantification shows greater alkane production under UW iGEM 2011's "optimized conditions" (see wiki for details)]]
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