Difference between revisions of "Part:BBa K590054"
Line 3: | Line 3: | ||
This part consists of [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590032 Acyl-ACP Reductase], ribosomal binding site, [https://parts.igem.org/Part:BBa_K590037 mutant Aldehyde Decarbonylase] under the control of high consititutive promoter for alkane production. | This part consists of [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590032 Acyl-ACP Reductase], ribosomal binding site, [https://parts.igem.org/Part:BBa_K590037 mutant Aldehyde Decarbonylase] under the control of high consititutive promoter for alkane production. | ||
− | |||
− | |||
===Usage and Biology=== | ===Usage and Biology=== | ||
+ | |||
+ | [[Image:Washington_Redesign_Data2.png|400px]] | ||
+ | |||
+ | When AAR and ADC are co-expressed, they produce odd chain length alkanes from the cell's fatty acid biosynthetic pathway. Alkane production is enhanced when growing expression strains using the growth conditions developed by the [http://2011.igem.org/Team:Washington 2011 University of Washington iGEM Team]. | ||
+ | |||
+ | [[Image:Washinton_AARADCGC-MS.png|left|400px|thumb|GC plot showing that alkanes are only produced when both ADC and AAR are expressed.]] | ||
+ | |||
+ | [[Image:Washinton_2011_Optimization_Quant.png|right|450px|thumb|GC-MS quantification shows greater alkane production under UW iGEM 2011's "optimized conditions" (see wiki for details)]] | ||
Revision as of 18:07, 16 September 2011
RrD_N25M-PSB1C3-High constitutive
This part consists of Acyl-ACP Reductase, ribosomal binding site, mutant Aldehyde Decarbonylase under the control of high consititutive promoter for alkane production.
Usage and Biology
When AAR and ADC are co-expressed, they produce odd chain length alkanes from the cell's fatty acid biosynthetic pathway. Alkane production is enhanced when growing expression strains using the growth conditions developed by the [http://2011.igem.org/Team:Washington 2011 University of Washington iGEM Team].
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 470
Illegal NheI site found at 493 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1584
Illegal XhoI site found at 2366 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 126
- 1000COMPATIBLE WITH RFC[1000]