Difference between revisions of "Part:BBa K525999:Design"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K525999 short</partinfo>
 
<partinfo>BBa_K525999 short</partinfo>
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===Design Notes===
 
===Design Notes===
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Assembled
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Firefly luciferase in Freiburg BioBrick assembly standard (RFC 25) as an enzymatic reporter domain
  
  
  
 
===Source===
 
===Source===
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* organism: ''Photinus pyralis''
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 +
* Created via PCR on <partinfo>I712019</partinfo> which was made from Promega's pGL3 plasmid
  
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BBa_I712019
 
  
 
===References===
 
===References===

Latest revision as of 17:23, 16 September 2011

Firefly -Luciferase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal AgeI site found at 1657
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 814


Design Notes

Firefly luciferase in Freiburg BioBrick assembly standard (RFC 25) as an enzymatic reporter domain


Source

  • organism: Photinus pyralis
  • Created via PCR on BBa_I712019 which was made from Promega's pGL3 plasmid


References