Difference between revisions of "Part:BBa K562009:Design"
Franksargent (Talk | contribs) m (→Design Notes) |
|||
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K562009 short</partinfo> | <partinfo>BBa_K562009 short</partinfo> | ||
| Line 7: | Line 6: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | Native PstI sites have been removed from pduB and pduU. There is an engineered BamHI site at the extreme 3' end, but please note that pduK contains two native BamHI sites that have not been removed. | + | Native PstI sites have been removed from pduB and pduU. There is an engineered BamHI site at the extreme 3' end, but please note that pduK contains two native BamHI sites that have not been removed. Be aware that pduK initiates at a GUG codon. |
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
Latest revision as of 12:48, 16 September 2011
Salty_PduABTUNJK BMC Components
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1898
Illegal BamHI site found at 3058
Illegal BamHI site found at 3197
Illegal BamHI site found at 3291 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1441
Illegal NgoMIV site found at 2685
Illegal AgeI site found at 1426
Illegal AgeI site found at 2040
Illegal AgeI site found at 2952
Illegal AgeI site found at 2988 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Native PstI sites have been removed from pduB and pduU. There is an engineered BamHI site at the extreme 3' end, but please note that pduK contains two native BamHI sites that have not been removed. Be aware that pduK initiates at a GUG codon.
Source
Derived from Salmonella enterica serovar Typhimurium LT2 genomic sequence.