Difference between revisions of "Part:BBa K562000:Design"
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===References=== | ===References=== | ||
| + | Jack RL, Sargent F, Berks BC, Sawers G, Palmer T. Constitutive expression of Escherichia coli tat genes indicates an important role for the twin-arginine translocase during aerobic and anaerobic growth. J Bacteriol. 2001 Mar;183(5):1801-4. | ||
Latest revision as of 08:58, 16 September 2011
Eco_Ptat
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 98
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The clone carries a BamHI restriction site at its 3' end (increasing the length of the part to 103 bp). If you use this site for cloning, and place a BamHI site (or similar 6-nucleotide site with compatible ends) immediately upsteam of the ATG start codon of your gene of interest, then this places your ATG in exactly the correct position to be initiated from the tatA RBS within the promoter region.
Source
This is from the Escherichia coli K-12 MG1655 genomic sequence.
References
Jack RL, Sargent F, Berks BC, Sawers G, Palmer T. Constitutive expression of Escherichia coli tat genes indicates an important role for the twin-arginine translocase during aerobic and anaerobic growth. J Bacteriol. 2001 Mar;183(5):1801-4.