Difference between revisions of "Part:BBa K590032"

Revision as of 03:02, 16 September 2011

Acyl-ACP Reductase (AAR) - Synechococcus elongatus PCC7942

This part encodes an enzyme that reduces cellular fatty acyl-ACPs, from the bacterial fatty acid biosynthesis pathway, into fatty aldehydes. Use together with Aldehyde Decarbonylase (ADC) to generate alkanes from acyl-ACPs. This part has been codon-optimized for E. coli.

Usage and Biology

When they are expressed together, AAR and ADC produce alkanes from the cell's fatty acid biosynthetic pathway [http://2011.igem.org/Team:Washington 2011 University of Washington iGEM Team] worked out conditions to grow E. coli containing AAR and ADC to generate alkanes.

GC plot showing that alkanes are only produced when both ADC and AAR are expressed.

GC-MS quantification shows greater alkane production under UW iGEM 2011's "optimized conditions" (see wiki for details)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 1060
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 8: / Line 8: @@
 ===Usage and Biology===
-The [http://2011.igem.org/Team:Washington 2011 University of Washington iGEM Team] worked out conditions to grow ''E. coli'' containing AAR and ADC to generate alkanes.
+When they are expressed together, AAR and ADC produce alkanes from the cell's fatty acid biosynthetic pathway [http://2011.igem.org/Team:Washington 2011 University of Washington iGEM Team] worked out conditions to grow ''E. coli'' containing AAR and ADC to generate alkanes.
 [[Image:Washinton_AARADCGC-MS.png|left|400px|thumb|GC plot showing that alkanes are only produced when both ADC and AAR are expressed.]]