Difference between revisions of "Part:BBa K590032"
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| + | [[Image:Washinton_AARADCGC-MS.png|right|400px|thumb|GC plot showing that alkanes are only produced when both ADC and AAR are expressed.]] | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 02:50, 16 September 2011
Acyl-ACP Reductase (AAR) - Synechococcus elongatus PCC7942
This part encodes an enzyme that reduces cellular fatty acyl-ACPs, from the bacterial fatty acid biosynthesis pathway, into fatty aldehydes. Use together with Aldehyde Decarbonylase (ADC) to generate alkanes from acyl-ACPs. This part has been codon-optimized for E. coli.
Usage and Biology
The [http://2011.igem.org/Team:Washington 2011 University of Washington iGEM Team] worked out conditions to grow E. coli containing AAR and ADC to generate alkanes.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1060
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]