Difference between revisions of "Part:BBa K515107"
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<p><b>Description</b></p> | <p><b>Description</b></p> | ||
| − | <p>Coding sequence <a href="https://parts.igem.org/Part:BBa_K515007">BBa_K515007</a>, under the control of repressible promoter TetR <a href="https://parts.igem.org/Part:BBa_R0040">BBa_R0040</a>. Coding sequence <a href="https://parts.igem.org/Part:BBa_K515007">BBa_K515007</a> codes for Dendra a photoconvertible reporter protein. In its natural state, it is excited and emits at 486 and 505nm, respectively. This appears green and therefore in its natural state Dendra can be used as GFP reporter. However it can be irreversibly converted to be excited at 558nm and consequently emit at 575nm by single-photon stimulation. After conversion reporter appears red and can be observed as RFP. Conversion can be efficiently achieved at wavelengths of both 488 and 405nm (Gurskaya et al., 2006). However, the cytotoxic wavelength of 405 converts Dendra more efficiently (data not shown).</p> | + | <p>Coding sequence <a href="https://parts.igem.org/Part:BBa_K515007">BBa_K515007</a>, under the control of repressible promoter TetR <a href="https://parts.igem.org/Part:BBa_R0040">BBa_R0040</a> with RBS <a href="https://parts.igem.org/Part:BBa_R0034">BBa_B0034</a>. Coding sequence <a href="https://parts.igem.org/Part:BBa_K515007">BBa_K515007</a> codes for Dendra a photoconvertible reporter protein. In its natural state, it is excited and emits at 486 and 505nm, respectively. This appears green and therefore in its natural state Dendra can be used as GFP reporter. However it can be irreversibly converted to be excited at 558nm and consequently emit at 575nm by single-photon stimulation. After conversion reporter appears red and can be observed as RFP. Conversion can be efficiently achieved at wavelengths of both 488 and 405nm (Gurskaya et al., 2006). However, the cytotoxic wavelength of 405 converts Dendra more efficiently (data not shown).</p> |
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Revision as of 22:25, 15 September 2011
Composite p(tetR) - Dendra2
Description
Coding sequence BBa_K515007, under the control of repressible promoter TetR BBa_R0040 with RBS BBa_B0034. Coding sequence BBa_K515007 codes for Dendra a photoconvertible reporter protein. In its natural state, it is excited and emits at 486 and 505nm, respectively. This appears green and therefore in its natural state Dendra can be used as GFP reporter. However it can be irreversibly converted to be excited at 558nm and consequently emit at 575nm by single-photon stimulation. After conversion reporter appears red and can be observed as RFP. Conversion can be efficiently achieved at wavelengths of both 488 and 405nm (Gurskaya et al., 2006). However, the cytotoxic wavelength of 405 converts Dendra more efficiently (data not shown).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]