Difference between revisions of "Part:BBa K537002:Design"

 
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===Source===
 
===Source===
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The cheZ sequence was PCR amplified from E.coli strain DH5α where the forward primers in the reaction contained the sequence for the theophylline riboswitch.  
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The CheZ gene was isolated from genomic DNA using PCR where the forward primers in the reaction contained the sequence for the theophylline riboswitch.  
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The riboswitch clone 12.1 is derived from Lynch and Gallivan NAR 2009.
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===References===
 
===References===

Revision as of 17:01, 15 September 2011

Theophylline Riboswitch 2-CheZ


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The effectiveness of the theophylline riboswitch is determined by the ability of the RNA 'loop' to hide the RBS preventing translation. The sequence of bases within the riboswitch will determine the formation of the loop and its leakiness.



Source

The cheZ sequence was PCR amplified from E.coli strain DH5α where the forward primers in the reaction contained the sequence for the theophylline riboswitch. The riboswitch clone 12.1 is derived from Lynch and Gallivan NAR 2009.

References