Difference between revisions of "Part:BBa K537001:Design"
(→Design Notes) |
|||
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K537001 short</partinfo> | <partinfo>BBa_K537001 short</partinfo> | ||
| Line 7: | Line 6: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | The effectiveness of the theophylline riboswitch is determined by the ability of the RNA 'loop' to hide the RBS preventing translation. The sequence of bases within the riboswitch will determine the formation of the loop and its leakiness. | + | The effectiveness of the theophylline riboswitch is determined by the ability of the RNA 'loop' to hide the RBS preventing translation. The sequence of bases within the riboswitch will determine the formation of the loop and its leakiness. The riboswitch and its adjacent CheZ coding region are considered together and should be cloned together in order to overcome the problems previously encountered with the synthetic theophylline riboswitches submitted by teams Lethbridge 2007, Lethbridge 2009, and NYMU Taipei 2010. |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
Revision as of 16:46, 15 September 2011
Theophylline Riboswitch 1-CheZ
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The effectiveness of the theophylline riboswitch is determined by the ability of the RNA 'loop' to hide the RBS preventing translation. The sequence of bases within the riboswitch will determine the formation of the loop and its leakiness. The riboswitch and its adjacent CheZ coding region are considered together and should be cloned together in order to overcome the problems previously encountered with the synthetic theophylline riboswitches submitted by teams Lethbridge 2007, Lethbridge 2009, and NYMU Taipei 2010.
Source
The CheZ gene was isolated from genomic DNA using PCR where the forward primers in the reaction contained the sequence for the theophylline riboswitch.