Difference between revisions of "Part:BBa K525998"
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| − | + | T7 promoter and RBS. The T7 promoter does not work with the RNA polymerase from ''Escherichia coli'' but with the RNA polymerase from the T7 phage. To express BioBricks under the control of a T7 promoter, ''E. coli'' carrying a T7 polymerase gene have to be used (e.g. BL21(DE3) or KRX). | |
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Revision as of 13:39, 15 September 2011
Promoter T7 and RBS
T7 promoter and RBS. The T7 promoter does not work with the RNA polymerase from Escherichia coli but with the RNA polymerase from the T7 phage. To express BioBricks under the control of a T7 promoter, E. coli carrying a T7 polymerase gene have to be used (e.g. BL21(DE3) or KRX).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]