Difference between revisions of "Part:BBa K568001"
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<partinfo>BBa_K568001 short</partinfo>
 
<partinfo>BBa_K568001 short</partinfo>
  
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This logical gate is based on amber stop-codon suppression via the non-canonical tRNA supD (<html><a href="https://parts.igem.org/Part:BBa_K228001">BBa_K228001</a></html>). The blue light sensor (<html><a href="https://parts.igem.org/Part:BBa_K238013">BBa_K238013</a></html>) induces the expression of a mRNA coding for a T7-polymerase with an amber mutation(<html><a href="https://parts.igem.org/Part:BBa_K228000">BBa_K228000</a></html>), that can only be translated by ribosomes if the correct supD tRNA is present. The supD tRNA is expressed when the red light sensor(<html><a href="https://parts.igem.org/Part:BBa_K568000">BBa_K568000</a></html>) is induced. Thus, only cells which receive both signals produce the desired substances.
+
This logical gate is based on amber stop-codon suppression via the non-canonical tRNA supD (<html><a href="https://parts.igem.org/Part:BBa_K228001">BBa_K228001</a></html>). The blue light sensor (<html><a href="https://parts.igem.org/Part:BBa_K238013">BBa_K238013</a></html>) induces the expression of a mRNA coding for a T7-polymerase with an amber mutation (<html><a href="https://parts.igem.org/Part:BBa_K228000">BBa_K228000</a></html>), that can only be translated by ribosomes if the correct supD tRNA is present. The supD tRNA is expressed when the red light sensor (<html><a href="https://parts.igem.org/Part:BBa_K568000">BBa_K568000</a></html>) is induced. Thus, only cells which receive both signals produce the desired substances.
  
  

Revision as of 21:08, 14 September 2011

Optogenetical AND-Gate red/blue light

This logical gate is based on amber stop-codon suppression via the non-canonical tRNA supD (BBa_K228001). The blue light sensor (BBa_K238013) induces the expression of a mRNA coding for a T7-polymerase with an amber mutation (BBa_K228000), that can only be translated by ribosomes if the correct supD tRNA is present. The supD tRNA is expressed when the red light sensor (BBa_K568000) is induced. Thus, only cells which receive both signals produce the desired substances.



Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 644
    Illegal XhoI site found at 1786
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 971
    Illegal AgeI site found at 3817
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3820


Functional Parameters

n/aOptogenetical AND-Gate red/blue light