Difference between revisions of "Part:BBa K568001"

Revision as of 21:05, 14 September 2011

Optogenetical AND-Gate red/blue light

This logical gate is based on amber stop-codon suppression via the non-canonical tRNA supD(Ba_K228001). The blue light sensor(BBa_K238013) induces the expression of a mRNA coding for a T7-polymerase with an amber mutation(BBa_K228000), that can only be translated by ribosomes if the correct supD tRNA is present. The supD tRNA is expressed when the red light sensor(<a href="https://parts.igem.org/Part:BBa_K568000">aaaaaa</a>) is induced. Thus, only cells which receive both signals produce the desired substances.

Usage and Biology

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 644
Illegal XhoI site found at 1786
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 971
Illegal AgeI site found at 3817
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 3820

Functional Parameters

n/a	Optogenetical AND-Gate red/blue light

Revision as of 21:00, 14 September 2011 (view source) Thorsten mueller (Talk \| contribs) ← Older edit		Revision as of 21:05, 14 September 2011 (view source) Thorsten mueller (Talk \| contribs) Newer edit →
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	<partinfo>BBa_K568001 short</partinfo>		<partinfo>BBa_K568001 short</partinfo>

−	This logical gate is based on amber stop-codon suppression via the non-canonical tRNA supD. The blue light sensor induces the expression of a mRNA coding for a T7-polymerase with an amber mutation, that can only be translated by ribosomes if the correct supD tRNA is present. The supD tRNA is expressed when the red light sensor is induced. Thus, only cells which receive both signals produce the desired substances.	+	This logical gate is based on amber stop-codon suppression via the non-canonical tRNA supD(Ba_K228001). The blue light sensor(BBa_K238013) induces the expression of a mRNA coding for a T7-polymerase with an amber mutation(BBa_K228000), that can only be translated by ribosomes if the correct supD tRNA is present. The supD tRNA is expressed when the red light sensor(<a href="https://parts.igem.org/Part:BBa_K568000">aaaaaa</a>) is induced. Thus, only cells which receive both signals produce the desired substances.
		+
		+