Difference between revisions of "Production/Competent Cells"
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<tr><th>Batch <th>Strain <th>Maker <th>Date Started <th>Temp, RPM <th>Incubation (hr) <th>Culture Volume <th>Culture OD (600nm) <th>Competent OD (600nm) <th>Cell Count (AVG) <th>Efficiency <th>250ul Aliquots <th>500ul Aliquots <th>1ml Aliquots <th>96w Plate</tr>
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<tr><th>Batch <th>Strain <th>Maker <th>Date Started <th>Temp, RPM <th>Incubation (hr) <th>Culture Volume <th>Culture OD (600nm) <th>Competent OD (600nm) <th>Cell Count (AVG) <th>Efficiency <th>250ul Aliquots <th>500ul Aliquots <th>1ml Aliquots <th>96w Plate <th>Checklist pdf</tr>
  
 
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<tr style="background-color:#C0C0C0;"><td>Expected Values<td><td><td><td>20C<td>16<td><td>0.3 or lower
 
<tr style="background-color:#C0C0C0;"><td>Expected Values<td><td><td><td>20C<td>16<td><td>0.3 or lower
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<td>1 - 1.5<td>100 - 400<td>5*10^8 - 5*10^9<td><td><td><td></tr>
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<td>1 - 1.5<td>100 - 400<td>5*10^8 - 5*10^9<td><td><td><td><td></tr>
  
 
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<tr><td>001 <td>NEB 10b <td>VS+KN <td>8/17/11 <td>21C, 250rpm <td>16 <td>500ml <td>0.4 <td>1.35 <td>67,88,112 (89) <td>1.335*10^8 <td>20 <td>20 <td>10 <td>0</tr>
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<tr><td>001 <td>NEB 10b <td>VS+KN <td>8/17/11 <td>21C, 250rpm <td>16 <td>500ml <td>0.4 <td>1.35 <td>67,88,112 (89) <td>1.335*10^8 <td>20 <td>20 <td>10 <td>0 <td>NA</tr>
 
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<tr><td>002 <td>NEB 10b <td>VS+KN <td>9/12/11 <td>20C, 250rpm <td>16 <td>500ml <td>0.40 <td> 1.12 <td> ??, ??, ?? (??) <td>?? <td> ?? <td> 0 <td> 0 <td>4</tr>
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<tr><td>002 <td>NEB 10b <td>VS+KN <td>9/12/11 <td>20C, 250rpm <td>16 <td>500ml <td>0.40 <td> 1.12 <td> ??, ??, ?? (??) <td>?? <td> ?? <td> 0 <td> 0 <td>4 <td>??</tr>
 
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<tr><td>003 <td>NEB 10b <td>VS+KN <td>9/12/11 <td>20C, 250rpm <td>16 <td>500ml <td>0.23 <td> 0.71 <td> ??, ??, ?? (??) <td>?? <td> ?? <td> 0 <td> 0 <td>4</tr>
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<tr><td>003 <td>NEB 10b <td>VS+KN <td>9/12/11 <td>20C, 250rpm <td>16 <td>500ml <td>0.23 <td> 0.71 <td> ??, ??, ?? (??) <td>?? <td> ?? <td> 0 <td> 0 <td>4 <td>??</tr>
 
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Revision as of 15:35, 14 September 2011


Batch Summary

Use this page to keep track of competent cell production. See the competent cell protocol for more information.

Batch Strain Maker Date Started Temp, RPM Incubation (hr) Culture Volume Culture OD (600nm) Competent OD (600nm) Cell Count (AVG) Efficiency 250ul Aliquots 500ul Aliquots 1ml Aliquots 96w Plate Checklist pdf
Expected Values20C160.3 or lower 1 - 1.5100 - 4005*10^8 - 5*10^9
001 NEB 10b VS+KN 8/17/11 21C, 250rpm 16 500ml 0.4 1.35 67,88,112 (89) 1.335*10^8 20 20 10 0 NA
002 NEB 10b VS+KN 9/12/11 20C, 250rpm 16 500ml 0.40 1.12 ??, ??, ?? (??) ?? ?? 0 0 4 ??
003 NEB 10b VS+KN 9/12/11 20C, 250rpm 16 500ml 0.23 0.71 ??, ??, ?? (??) ?? ?? 0 0 4 ??

Batch Notes

001

This is the first batch attempting to fully standardize competent cell production. Also Kitwa's first batch! And a resounding success!

The efficiency test/test transformation used a single 250ul aliquot (randomly chosen). Three transformations were done using our standard template and protocol. Important: In following productions we will test the first and last of the aliquots.

002

OD600nm value at 15.5 hours was 0.26, and cells were re-placed in the shaker for another 0.5 hours. OD value afterward was 0.40.

003

OD600nm value at 15.5 hours was 0.16, and cells were re-placed in the shaker for another 0.5 hours. OD value afterward was 0.23.