Difference between revisions of "Part:BBa K608101:Design"
Juliimapril (Talk | contribs) (→Source) |
|||
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K608101 short</partinfo> | <partinfo>BBa_K608101 short</partinfo> | ||
| Line 15: | Line 14: | ||
It was PCR-amplified from the genomic sequence of Synechocystis sp. PCC 6803. | It was PCR-amplified from the genomic sequence of Synechocystis sp. PCC 6803. | ||
The following primers were used: | The following primers were used: | ||
| − | XbaI_RBS_ccaR_up, TATTCTAGATTCACACAGGAAACCTCACTAATGAGAATTCTTTTAGTGGAGGATG | + | XbaI_RBS_ccaR_up, TATTCTAGATTCACACAGGAAACCTCACTAATGAGAATTCTTTTAGTGGAGGATG and |
ccaR_SpeI_PstI_dw,GCCGGACTGCAGCGGCCGCTACTAGTCTAGTTTTTCCCTTGGCACAAAG | ccaR_SpeI_PstI_dw,GCCGGACTGCAGCGGCCGCTACTAGTCTAGTTTTTCCCTTGGCACAAAG | ||
===References=== | ===References=== | ||
Revision as of 10:08, 14 September 2011
CcaR, green light response regulator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 380
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We deleted an EcoRI restriction site in CcaR, in order to make it compatible to the iGEM standart.
Source
It was PCR-amplified from the genomic sequence of Synechocystis sp. PCC 6803. The following primers were used: XbaI_RBS_ccaR_up, TATTCTAGATTCACACAGGAAACCTCACTAATGAGAATTCTTTTAGTGGAGGATG and ccaR_SpeI_PstI_dw,GCCGGACTGCAGCGGCCGCTACTAGTCTAGTTTTTCCCTTGGCACAAAG