Difference between revisions of "Part:BBa K515107"
Line 2: Line 2:
 
<partinfo>BBa_K515107 short</partinfo>
 
<partinfo>BBa_K515107 short</partinfo>
  
−
-
+
<p><b>Description</b></p>
−
 
+
<p>Biobrick <a href="https://parts.igem.org/Part:BBa_K515007">BBa_K515007</a>, a translational subunit under the control of promoter  Dendra is a photoconvertible protein. In its natural state, it is excited and emits at 486 and 505nm, respectively. It can be converted to be excited at 558nm and consequently emit at 575nm by single-photon stimulation. Conversion can be efficiently achieved at wavelengths of both 488 and 405nm (Gurskaya et al., 2006). However, the cytotoxic wavelength of 405 converts Dendra more efficiently (data not shown).</p>
−
 
+
−
===Usage and Biology===
+
−
Dendra is a photoconvertible protein. In its natural state, it is excited and emits at 486 and 505nm, respectively. It can be converted to be excited at 558nm and consequently emit at 575nm by single-photon stimulation. Conversion can be efficiently achieved at wavelengths of both 488 and 405nm (Gurskaya et al., 2006). However, the cytotoxic wavelength of 405 converts Dendra more efficiently (data not shown).
+
 
<!-- -->
 
<!-- -->
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 06:28, 14 September 2011

Composite p(tetR) - Dendra2

Description

Biobrick <a href="https://parts.igem.org/Part:BBa_K515007">BBa_K515007</a>, a translational subunit under the control of promoter Dendra is a photoconvertible protein. In its natural state, it is excited and emits at 486 and 505nm, respectively. It can be converted to be excited at 558nm and consequently emit at 575nm by single-photon stimulation. Conversion can be efficiently achieved at wavelengths of both 488 and 405nm (Gurskaya et al., 2006). However, the cytotoxic wavelength of 405 converts Dendra more efficiently (data not shown).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterisation

Dendra can be efficiently converted using confocal microscopy.