Difference between revisions of "Part:BBa K678000"
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DMKP-P6 is a newly identified constitutive ''Aspergillus nidulans'' promoter of medium strength.
 
DMKP-P6 is a newly identified constitutive ''Aspergillus nidulans'' promoter of medium strength.
  
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[[Image:Img0003-51.jpg|frame|none|Figure 1: As can be seen on the plate we have two positive controls the strong PgpdA 0.5kb and PgpdA 1.0kb promoters and a negative control that does not posses the lacZ gene. By comparing the intensities of the blue color we can see that DMKP-P6 is most likely a promoter of medium strength. To further investigate this we performed a β-galactosidase assay.]]
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[[Image:Dtu2b3.jpg|frame|none|Figure 1: As can be seen on the plate we have two positive controls the strong PgpdA 0.5kb and PgpdA 1.0kb promoters and a negative control that does not posses the lacZ gene. By comparing the intensities of the blue color we can see that DMKP-P6 is most likely a promoter of medium strength. To further investigate this we performed a β-galactosidase assay.]]
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https://static.igem.org/mediawiki/igem.org/0/07/Img0003-51.jpg
 
  
 
https://static.igem.org/mediawiki/igem.org/c/c4/ActivityDMKP.png
 
https://static.igem.org/mediawiki/igem.org/c/c4/ActivityDMKP.png

Revision as of 12:47, 13 September 2011

DMKP-P6, Aspergillus nidulans promoter

DMKP-P6 is a newly identified constitutive Aspergillus nidulans promoter of medium strength.

Figure 1: As can be seen on the plate we have two positive controls the strong PgpdA 0.5kb and PgpdA 1.0kb promoters and a negative control that does not posses the lacZ gene. By comparing the intensities of the blue color we can see that DMKP-P6 is most likely a promoter of medium strength. To further investigate this we performed a β-galactosidase assay.


ActivityDMKP.png

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 23
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 23
    Illegal NheI site found at 473
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 23
    Illegal XhoI site found at 122
    Illegal XhoI site found at 132
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 23
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 23
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 212
    Illegal BsaI.rc site found at 499